Urinary tract is the most common site of infection after the respiratory tract. Various factors play a role in causing infection in the urinary tract, and in 80% of cases, Escherichia coli bacteria is the cause of infection. In the meantime, increasing antibiotic resistance of bacteria leads to treatment failure and disease recurrence. Due to the different pattern of antibiotic resistance of bacteria in each region, it is necessary to study and check the antibiotic resistance of bacteria. The aim of this study was to determine the antibiotic resistance of bacteria in urine samples of dogs in Babol city. To do so, 100 samples of dogs with urinary tract infection referred to private clinics of Babol city were examined. One hour after collecting the samples, 0.01 ml of urine was cultured directly on the blood agar medium using a standard loop and incubated at 35-37^oC for 24-48 hours. After that, the antibiogram was performed and finally the area of non-growth around the antibiotic discs was measured. Our findings showed that gram-negative bacteria were the most abundant in this study, indicating the most resistant to clindamycin (92.59%), penicillin (87.04%) and amoxicillin (79.63%). The least resistant to amikacin and gentamicin (12.96%), and trimethoprim (28.52%) was observed. According to the results, it is necessary to perform a precise antibiogram test using reliable discs before prescribing the drug, as well as rationally prescribing antibiotics and not arbitrarily taking them.