Caspian Journal of Veterinary Sciences

Caspian Journal of Veterinary Sciences

Antibiotic resistance in isolated bacteria from dogs with urinary tract infection in Babol city

Document Type : Original Article

Authors
Fatemeh Hadinejad 1
Fatemeh zahra Gharib 2
1 DVM graduated, Babol Branch, Islamic Azad University, Babol, Iran.
2 Department of Clinical Sciences, Babol Branch, Islamic Azad University, Babol, Iran.
10.22034/cjvs.2025.491105.1035
Abstract
Urinary tract is the most common site of infection after the respiratory tract. Various factors play a role in causing infection in the urinary tract, and in 80% of cases, Escherichia coli bacteria is the cause of infection. In the meantime, increasing antibiotic resistance of bacteria leads to treatment failure and disease recurrence. Due to the different pattern of antibiotic resistance of bacteria in each region, it is necessary to study and check the antibiotic resistance of bacteria. The aim of this study was to determine the antibiotic resistance of bacteria in urine samples of dogs in Babol city. To do so, 100 samples of dogs with urinary tract infection referred to private clinics of Babol city were examined. One hour after collecting the samples, 0.01 ml of urine was cultured directly on the blood agar medium using a standard loop and incubated at 35-37oC for 24-48 hours. After that, the antibiogram was performed and finally the area of non-growth around the antibiotic discs was measured. Our findings showed that gram-negative bacteria were the most abundant in this study, indicating the most resistant to clindamycin (92.59%), penicillin (87.04%) and amoxicillin (79.63%). The least resistant to amikacin and gentamicin (12.96%), and trimethoprim (28.52%) was observed. According to the results, it is necessary to perform a precise antibiogram test using reliable discs before prescribing the drug, as well as rationally prescribing antibiotics and not arbitrarily taking them.
Keywords
Antibiotic resistance
Babol
Dog
Urinary tract infection
Subjects
Bahadoran, S; Zamani Moghadam, A; Homai Borujeni, S; Borjian, S and Borujeni, S (2008). Evaluation of Escherichia coli antibiotic resistance in herds referred to Shahrekord University Veterinary Clinic. 15th Iranian Veterinary Congress 2008.
Ball, KR; Rubin, JE; Chirino-Trejo, M and Dowling, PM (2008). Antimicrobial resistance and prevalence of canine uropathogens at the Western College of Veterinary Medicine Veterinary Teaching Hospital, 2002–2007. Can. Vet. J., 49: 985.
Black, DM; Rankin, SC and King, LG (2009). Antimicrobial therapy and aerobic bacteriologic culture patterns in canine intensive care unit patients: 74 dogs (January-June 2006). J. Vet. Emerg. Crit. Care.,19: 489-495.
Blount, ZD (2015). The unexhausted potential of E. coli. Elife., 4: e05826.
Farhadi Touli, L; Yazdanfar, S and Faramarzi, S (2012). Isolation and identifying gram-negative bacteria in urine samples of rasht's razi hospital patients with urinary tract infection and determining their antimicrobial resistance pattern. Biotechnol. J. Environ. Microorganisms., 6: 45-53.
Grabem, M; Bjerklund-Johansen, T; Botto, H; Çek, M; Naber, K; Tenke, P and Wagenlehner, F (2015). Guidelines on urological infections. Eur. Assoc. Urol., 182: 237-257.
Hall, JL; Holmes, MA and Baines, SJ (2013). Prevalence and antimicrobial resistance of canine urinary tract pathogens. Vet. Rec., 173: 549.
Hernando, El; Vila, A; D'Ippolito, P; Rico, AJ; Rodon, J and Roura, X (2021). Prevalence and characterization of urinary tract infection in owned dogs and cats from Spain. Top. Companion Anim. Med., 43: 100512.
Jessen, LR; Sorensen, TM; Bjornvad, CR; Nielsen, SS and Guardabassi, L (2015). Effect of antibiotic treatment in canine and feline urinary tract infections: a systematic review. Vet. J., 203: 270-277.
Khoshbakht, R; Ajam, Z and Amiri, F (2019). Prevalence and antibiotic resistance of Staphylococcus aureus in dogs, cats and their owners in Amol city. The Second International Conference on New Technologies in Science 2019.
Ling, GV; Franti, CE; Johnson, DL and Ruby, AL (1998). Urolithiasis in dogs. III: Prevalence of urinary tract infection and interrelationsof infection, age, sex, and mineral composition. Am. J. Vet. Res., 59: 643-649.
 Mirmahdavi, F and Ahmadian, A (2001). Isolated bacteria from urinary tract infections and  their antibiotic sensitivity(1994-97). Med. J. Tabriz Univ. Med. Sci., 34: 53-58.
Namroodi, S; Staji, H and Sharafi, SV (2017). Survey on Salmonella contamination of Golden Jackals by microbiological culture methods and PCR in Golestan and Mazandaran Provinces. J. Vet. Res., 72: 269-276.
Olin, SJ and Bartges, JW (2022). Urinary tract jnfections treatment/comparative therapeutics. Vet. Clin. North Am. Small Anim. Pract., 52: 581-608.
Pour Mirbolok Jalali, E; Dadash Begi, M and Asad Pour, L (2020). Investigation on the bacterial of dog's hemorrhagic enteritis and determining their antibiotic resistance in Rasht veterinary clinics. J. Clin. Res. Large Anim., 9: 1-6.
Salehi, A; Hosseini, SM and Kazemi, S (2022). Antioxidant and anticarcinogenic potentials of propolis for dimethylhydrazine-induced colorectal cancer in wistar rats. BioMed. Res. Int.,  2022; 2022.
Seguin, MA; Vaden, SL; Altier, C; Stone, E and Levine, JF (2003). Persistent urinary tract infections and reinfections in 100 dogs (1989-1999). J. Vet. Int. Med., 17: 622-631.
Vahhabi, A; Hasani, A; Nahaei, M and Frajnia, S (2010). PCR assay for detection of vanA and vanB genes from intestinal carriers of vancomycin-resistant enterococci in three educational hospitals of Tabriz university of medical sciences. Iran. J. Med. Microbiol., 4: 58-65.
 Weaver, A and Pillinger, R (1977). Lowerurinary tract pathogens in the dog and their sensitivity to chemotherapeutic agents. Vet. Rec., 101: 77-79.
 
 
 
 
 
 
Caspian Journal of Veterinary Sciences
Volume 2, Issue 1
June 2025
Pages 37-41

  • Receive Date 27 November 2024
  • Revise Date 12 February 2025
  • Accept Date 12 February 2025