Caspian Journal of Veterinary Sciences

Caspian Journal of Veterinary Sciences

Detection of Listeria monocytogenes from fresh vegetables in Amol, northern Iran by PCR method

Document Type : Original Article

Authors
Rahem Khoshbakht 1
Emad Ahmadi Ara 1
Mojtaba Khosravi 1
Hani Mirzadeh 2
pouya Mohammad Taghipoor 2
Fatemeh Zahra Hosseinpour 2
1 Department of Pathobiology, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran; Zoonotic Diseases Research Group, Faculty of Veterinary Medicine, Amol University of Modern Technologies, Amol, Iran.
2 Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran.
10.22034/cjvs.2025.222396
Abstract
Bacterial contamination of food is an important threat to food safety, which negatively affects health, economy, and society's well-being. Listeria monocytogenes is one of the pathogenic bacteria whose contamination in food is continuously increasing. Fresh vegetables are one of the food groups that can serve as a reservoir for L. monocytogenes bacteria. The increased consumption of fresh vegetables in recent years has led to increased cases of gastrointestinal infections caused by L. monocytogenes. The purpose of this study was the molecular isolation and sequencing of L. monocytogenes bacteria in fresh vegetables offered in the markets of Amol and Babol cities, Mazandaran province, Iran. Coriander and oregano were obtained, and the samples were analyzed using polymerase chain reaction (PCR) to identify L. monocytogenes bacteria. Among the samples, five samples were positive for the presence of Listeria genus and two samples were positive for the presence of L. monocytogenes, which indicated the contamination of vegetables from Mazandaran with L. monocytogenes. Although the prevalence of L. monocytogenes in this study was small, the presence of any type of Listeria can indicate improper sanitary conditions in different stages of production until the supply of vegetables or the possible contamination of vegetables by other pathogens.
Keywords
Food safety
Fresh vegetables
Listeria monocytogenes
Polymerase chain reaction
Subjects
Al-Kharousi, ZS; Guizani, N; Al-Sadi, AM; Al-Bulushi, IM and Shaharoona, B (2016). Hiding in fresh fruits and vegetables: opportunistic pathogens may cross geographical barriers. Int. J. Microbiol., 2016: 4292417.
Amajoud, N; Leclercq, A; Soriano, J; Bracq-Dieye, H and El Maadoudi, M (2018). Prevalence of Listeria spp. and characterization of Listeria monocytogenes isolated from food products in Tetouan, Morocco. Food Contr., 84: 436-441.
Baert, L; Uyttendaele, M and Debevere, J (2008). Evaluation of viral extraction methods on a broad range of Ready-To-Eat foods with conventional and real-time RT-PCR for Norovirus GII detection. Int. J. Food Microbiol.,123: 101–108.
Bongiovanni, M; Cavallo, C; Barda, B; Strulak, L; Bernasconi, E and Cardia, A (2024). Clinical findings of Listeria monocytogenes infections with a special focus on bone localizations. Microorganisms, 12: 178.
Chen, JQ; Healey, S; Regan, P; Laksanalamai, P and Hu, Z (2017). PCR-based methodologies for detection and characterization of Listeria monocytogenes and Listeria ivanovii in foods and environmental sources. Food Sci. Hum. Wellness, 6: 39-59.
Elbehiry, A; Abalkhail, A; Marzouk, E; Elmanssury, AE; Almuzaini, AM; Alfheeaid, H; Alshahrani, MT; Huraysh, N; Ibrahem, M; Alzaben, F; Alanazi, F; Alzaben, M; Anagreyyah, SA; Bayameen, AM; Draz, A and Abu-Okail, A (2023). An overview of the public health challenges in diagnosing and controlling human foodborne pathogens. Vaccines, 11: 725.
Gopalakrishnan, DR (2019). Advantages and nutritional value of organic food on human health. Int. J. Trend Sci. Res. Develop., 3: 242-245.
Koutsoumanis, K; Alvarez-Ordóñez, A; Bolton, D; BoverCid, S; Chemaly, M; Davies, R; De Cesare, A; Herman, L; Hilbert, F; Lindqvist, R; Nauta, M; Peixe, L; Ru, G; Simmons, M; Skandamis, P; Suffredini, E; Jordan, K; Sampers, I; Wagner, M and Allende, A (2020). The public health risk posed by Listeria monocytogenes in frozen fruit and vegetables including herbs, blanched during processing. J. Eur. Food Safe. Author., 18: e06092.
Osek, J and Wieczorek, K (2022). Listeria monocytogeneshow this pathogen uses its virulence mechanisms to infect the hosts. Pathog., 11: 1491.
Santos, MI; Grácio, M; Silva, MC; Pedroso, L and Lima, A (2023). One health perspectives on food safety in minimally processed vegetables and fruits: from farm to fork. Microorganisms, 11: 2990.
Shamloo, E; Hosseini, H; Abdi Moghadam, Z; Halberg Larsen, M; Haslberger, A and Alebouyeh, M (2019). Importance of Listeria monocytogenes in food safety: a review of its prevalence, detection, and antibiotic resistance. Iran. J. Vet. Res., 20: 241–254.
Caspian Journal of Veterinary Sciences
Volume 2, Issue 1
June 2025
Pages 31-36

  • Receive Date 30 October 2024
  • Revise Date 09 November 2024
  • Accept Date 10 November 2024