In this study, leaf and flower samples from Khansar and Kerman ecotypes of Leonurus cardiaca were extracted with methanol and ethanol to analyze their phenolic content, antioxidant activity, and scolicidal efficacy against E. granulosus protoscoleces. Total phenolic content analysis revealed higher values in methanolic extracts, with Khansar leaf extracts yielding 37.6 ±1.2 mg GAE/g compared to Kerman's 31.1 ±1.4 mg GAL/g. Total flavonoid content showed a similar trend, with Khansar leaf methanolic extracts producing 21.6 ± 1.1 mg QE/g versus Kerman's 15.6 ± 1.1 mg QE/g. Total flavonol content analysis corroborated these findings, with Khansar leaf methanolic extracts yielding 7.5 ± 1.0 mg QE/g compared to Kerman's 6.6 ± 1.1 mg QE/g. DPPH radical scavenging assays demonstrated concentration-dependent antioxidant activity, with Khansar leaf methanolic extracts showing 62.2 ± 1.0% inhibition at 120 µg/ml, surpassing Kerman's 59.0 ± 1.2%. Ferric-reducing power assays further supported these results, with Khansar leaf methanolic extracts exhibiting an absorbance of 0.437 ± 0.005 at 400 µg/ml. Scolicidal activity against E. granulosus protoscoleces increased with concentration, with the Khansar ecotype achieving 100% mortality at 90 mg/ml after 30 min, and at 120 mg/ml after 20 min. The Kerman ecotype did not reach 100% mortality at 90 mg/ml concentration, but at 120 mg/ml, it achieved 100% mortality after 30 min. These results indicate that L. cardiaca extracts, particularly from Khansar and Kerman ecotypes, show promise as a potential treatment for E. granulosus infections.