Caspian Journal of Veterinary Sciences

Caspian Journal of Veterinary Sciences

Comparative analysis of scolicidal properties of two ecotypes of Leonurus cardiaca: implications for combating echinococcus granulosus

Document Type : Original Article

Authors
Mahmoud Kiani 1
Mojtaba Ranjbar 2
Ali Nikpay 3
1 Faculty of Medicinal Plants, Amol University of Special Modern Technologies, Amol, Iran
2 Faculty of Biotechnology, Amol University of Special Modern Technologies, Amol, Iran
3 Department of Pathobiology, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran
10.22034/cjvs.2024.210714
Abstract
In this study, leaf and flower samples from Khansar and Kerman ecotypes of Leonurus cardiaca were extracted with methanol and ethanol to analyze their phenolic content, antioxidant activity, and scolicidal efficacy against E. granulosus protoscoleces. Total phenolic content analysis revealed higher values in methanolic extracts, with Khansar leaf extracts yielding 37.6 ±1.2 mg GAE/g compared to Kerman's 31.1 ±1.4 mg GAL/g. Total flavonoid content showed a similar trend, with Khansar leaf methanolic extracts producing 21.6 ± 1.1 mg QE/g versus Kerman's 15.6 ± 1.1 mg QE/g. Total flavonol content analysis corroborated these findings, with Khansar leaf methanolic extracts yielding 7.5 ± 1.0 mg QE/g compared to Kerman's 6.6 ± 1.1 mg QE/g. DPPH radical scavenging assays demonstrated concentration-dependent antioxidant activity, with Khansar leaf methanolic extracts showing 62.2 ± 1.0% inhibition at 120 µg/ml, surpassing Kerman's 59.0 ± 1.2%. Ferric-reducing power assays further supported these results, with Khansar leaf methanolic extracts exhibiting an absorbance of 0.437 ± 0.005 at 400 µg/ml. Scolicidal activity against E. granulosus protoscoleces increased with concentration, with the Khansar ecotype achieving 100% mortality at 90 mg/ml after 30 min, and at 120 mg/ml after 20 min. The Kerman ecotype did not reach 100% mortality at 90 mg/ml concentration, but at 120 mg/ml, it achieved 100% mortality after 30 min. These results indicate that L. cardiaca extracts, particularly from Khansar and Kerman ecotypes, show promise as a potential treatment for E. granulosus infections.
Keywords
Antioxidant activity
Echinococcus granulosus
Leonurus cardiac
Phenolic compounds
Scolicidal activity
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Caspian Journal of Veterinary Sciences
Volume 1, Issue 2
December 2024
Pages 151-160

  • Receive Date 16 August 2024
  • Revise Date 14 September 2024
  • Accept Date 14 September 2024