Caspian Journal of Veterinary Sciences

Caspian Journal of Veterinary Sciences

Histopathological and biochemical evaluation of protective effects of lemongrass (Cymbopogon citratus) essential oil on 5-Fluorouracil-induced intestinal damage in murine model

Document Type : Original Article

Authors
Navideh Mirzakhani 1
Atefeh Araghi 2
Mohaddeseh Abouhoseini 3
1 Department of Pathobiology, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran
2 Department of Clinical Sciences, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran
3 Department of Basic Sciences, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran
10.22034/cjvs.2024.198853
Abstract
5-Fluoraracil (5-FU) is a common chemotherapy drug, but its toxicity is considered a big obstacle to successful cancer chemotherapy. Oxidative stress plays an important role in 5-FU toxicity. Plant compounds reduce the damage caused by oxidative stress. This study aimed to explore the potential protective effects of lemongrass essential oil (LG) on intestinal tissue damage caused by 5-FU. Forty-eight mice were divided into six groups including control, 5-FU (200 mg/kg, IP), 5-FU (200 mg/kg, IP along with LG (125, 250, and 500 mg/kg, PO), and LG alone (500 mg/kg, PO) for six days. On the seventh day, serum and intestinal tissue samples were collected. The histopathological analysis of the intestine tissues revealed that a single dose of 5-FU led to degenerative damage, destruction of the epithelium, shortened villi length, and edema in the small intestine. It also caused a significant increase in serum (malondialdehyde) MDA levels, and a notable reduction in superoxide dismutase (SOD) and catalase serum levels. Oral administration of LG, particularly at doses of 250 and 500 mg/kg, significantly mitigated the severity of these lesions and serum marker alterations. Importantly, the study observed that administering a high dose of LG did not induce any pathological effects in the intestine tissue or serum. LG oil offers protective effects against 5-FU-induced damage in the mice intestine and serum biochemical changes, which can help reduce oxidative damage caused by free radicals generated by 5-FU.
Keywords
Essence
5-Fluorouracil
Histopathology
Lemongrass
Small intestine
Subjects
Adeleke, A; Kasali, A and Debola, O (2001). Volatile leaf oil
constituents of Cymbopogon citratus Stapf. Flavour Fragr.
J., 16: 377-378.
Esmort, HC; David, RH and Dudley, IF (1998). Volatile
constituents of the essential oil of Cymbopogon citratus
stapf grown in Zambia. Flavour Fragr. J., 13: 29-30.
Fideles, LD; de Miranda, JA and Martins, CD (2020). Role
of rutin in 5-fluorouracil-induced intestinal mucositis:
prevention of histological damage and reduction of
inflammation and oxidative stress. Mol., 25: 2786-2789.
Gan, Y; Ai, G; Wu, J; Luo, H; Chen, L; Huang, Q; Wu, X; Xu,
N; Li, M; Su, Z and Liu, Y (2020). Patchouli oil
ameliorates 5-fluorouracil-induced intestinal mucositis in
rats via protecting intestinal barrier and regulating water
transport. J. Ethnopharmacol., 250: 112519.
Guimarães, LG; dasGraças Cardoso, M; Souza, PE; de
Andrade, J and Vieira, SS (2011). Antioxidant and
fungitoxic activities of the lemongrass essential oil and
citral. Rev. Cienc. Agron., 42: 464.
Hanahan, D and Weinberg, RA (2011). Hallmarks of cancer:
the next generation. Cell., 144: 646-674.
Iacopetta, B; Kawakami, K and Watanabe, T (2008).
Predicting clinical outcome of 5-fluorouracil-based
chemotherapy for colon cancer patients: is the CpG island
methylator phenotype the 5-fluorouracil-responsive
subgroup? Int. J. Clin. Oncol., 13: 498–503.
Krishnamurthi, SS and Macaron, C (2019). Management of
acute chemotherapy-related diarrhea. Up-to-date.
Accessed August. 2019; 5.
Lamberti, M; Porto, S; Marra, M; Zappavigna, S; Grimaldi, A; Feola, D; Pesce, D; Naviglio, S; Spina, A; Sannolo, N
and Caraglia, M (2012). 5-Fluorouracil induces
apoptosis in rat cardiocytes through intracellular
oxidative stress. J. Exp. Clin. Cancer Res., 31: 1-8.
Launay-Vacher, V; Oudard, S and Janus, N (2007).
Prevalence of renal insufficiency in cancer patients and
implications for anticancer drug management: the renal
insufficiency and anticancer medications (IRMA) study.
Cancer., 110: 1376.
Leocádio, PC; Antunes, MM and Teixeira, LG (2015). Larginine pretreatment reduces intestinal mucositis as
induced by 5-FU in mice. Nutr. Cancer., 67: 486-493.
Longley, DB; Harkin, DP and Johnston, PG (2003). 5-
fluorouracil: mechanisms of action and clinical strategies.
Nat. Rev. Cancer., 3: 330–338.
Lotfi, M; Kazemi, S and Shirafkan, F (2021). The protective
effects of quercetin nano-emulsion on intestinal
mucositis induced by 5-fluorouracil in mice. BBRC., 585:
75-81.
Manvitha, K and Bidya, B (2014). Review on
pharmacological activity of Cymbopogon citratus. Int. J.
Herb., 1: 5-7.
Mirzakhani, N; Farshid, AA; Tamaddonfard, E; Tehrani, A
and Imani, M (2020). Comparison of the effects of
hydroalcoholic extract of Capparis spinosa fruit, quercetin
and vitamin E on monosodium glutamate-induced
toxicity in rats. Vet. Res. Forum.,11: 127-134.
Numazawa, S; Sugihara, K; Miyake, S; Tomiyama, H; Hida,
A; Hatsuno, M; Yamamoto, M and Yoshida, T (2011).
Possible involvement of oxidative stress in 5‐
Fluorouracil‐mediated myelosuppression in mice. Clin.
Toxicol.,108: 40-45.
Parker, WB and Cheng, YC (1990). Metabolism and
mechanism of action of 5-fluorouracil. Pharmacol. Ther.,
48: 381–395.
Robbing, SRJ (1983). Selected Markets for the Essential oils of
lemongrass, Citronella and Eucalyptus. Tropical Products
Institute Report. G171. London Press. PP: 13.
Rosner, MH and Perazella, MA (2017). Acute kidney injury
in patients with cancer. NEJM., 376: 1770-1781.
Ross, IA (2005). Medicinal Plants of the World. 2th Ed.
Humana Press. PP: 263-281.
Rtibi, K; Selmi, S; Grami, D; Amri, M; Sebai, H and
Marzouki, L (2018). Contribution of oxidative stress in
acute intestinal mucositis induced by 5 fluorouracil (5-
FU) and its pro-drug capecitabine in rats. Toxicol. Mech.
Methods., 28: 262-267.
Shi, C; Zhao, X; Liu, Z; Meng, R; Chen, X and Guo, N (2016).
Antimicrobial, antioxidant, and antitumor activity of
epsilon-poly-L-lysine and citral, alone or in combination.
FNR., 60: 31891.
Telloni, SM (2017). Tumor staging and grading: A primer.
Methods. Mol. Biol., 1606: 1-17.
Tiwari, M; Dwivedi, UN and Kakkar, P (2010). Suppression
of oxidative stress and pro-inflammatory mediators by
Cymbopogon citratus D. Stapf extract in
lipopolysaccharide stimulated murine alveolar
macrophages. Food Chem. Toxicol., 48: 2913-2919.
Tizianna Baratta, M and Giuseppe, R (1998). Antimicrobial
and antioxidant properties of some commercial essential
oils. Flavour Fragr. J., 13: 235-244.
Uchida, NS; Silva-Filho, SE and Aguiar, RP (2017).
Protective effect of Cymbopogon citratus essential oil in
experimental model of acetaminophen-induced liver
injury, Am. J. Chin. Med., 45: 1–18.
Yazdani, D; Rezazadeh, S and Shahabi, NA (2004). Volatile
oil constituents of Lemon grass (Cymbopogon citratus
Stapf) cultivated in north of IRAN. J. Med. plant., 3: 69-74.
Zheng, H; Gao, J and Man, SH (2019). The protective effects
of Aquilariae Lignum Resinatum extract on 5-Fuorouracilinduced intestinal mucositis in mice. Phytomed., 54: 308-
317.
Caspian Journal of Veterinary Sciences
Volume 1, Issue 1
June 2024
Pages 63-68

  • Receive Date 14 April 2024
  • Revise Date 03 June 2024
  • Accept Date 03 June 2024